ExCel Matrix Biological Devices (P) Ltd.
First and foremost challenge in tissue engineering today is an appropriate cell-interactive engineering biomaterial. 3D cell culture is the first step towards engineered tissues and regenerative medicine research and product development involving a porous scaffold of cell-interactive biomaterial.
Concepts of 3D cell culture are well established and its relative advantages over conventional cell culture well appreciated. However, current methods for 3D cell culture are neither convenient, nor cheap. Convenience and cost factor are discouraging many potential users and slowing down acceptance of 3D cell culture as standard research tool. Conventional cell culture applications are limited in future as it can not be adapted to the needs of modern cell biology research, diagnostic bioassays or tissue engineering.
We have developed a unique biomaterial technology called ECM Analog®, with customizable cell interactivity and engineering properties that has been developed since last three years for similar purposes.
Our ability to handle protein and glycans as key building blocks for generation of artificial tissues is perhaps unsurpassed. We have been able to organize these and other synthetic/ natural molecules on a nanometer scale using our proprietary technologies. While these, highly ordered nano-assemblies provide real tissue specific environment to the cells to be cultured, it also provide sufficient engineering versatility for scale-up manufacturing, automation and robust biomaterial handling.
Extracellular matrix based biomaterials (e.g. collagen, small intestine submucosa, amniotic membrane etc.) technologies are notoriously difficult to adapt to manufacturing. There is no biomaterial platform available commercially for tracking, retrieval and analysis of transplanted cells in vivo. As many aspects of tissue microenvironment can not be replicated in vitro, in vivo cell culture is necessary at times.
ECM Analog® can be customized for tissue specific requirements and manufacturing environment conveniently. ECM Analog® technology can be used for immobilization of cells during in vivo incubation. We have created 3D cell culture devices for research & development in tissue engineering though our patented ECM Analog® technology. These devices are combination of superior design, convenience, application range and IP protection unlike any competitor product worldwide.
We have demonstrated following key steps required for a successful tissue engineering product development;
- Porous scaffold preparation
- Isolation of tissue specific ECM w/o latent infectious agents
- Controlled nano-assembly of extracellular matrix
- Avoiding toxic substance normally used for scaffold preparation
- Pore morphology customization (porosity and pore diameter)
- Shaping and sizing the scaffold in any desirable manner … see figure
- In vitro cell culture on ECM Analog
- Culture of a variety of cell lines
- Primary culture of animals and human cells
- In vitro biodegradation time
- In vivo biocompatibility of ECM Analog … see figure
- Biodegradation time of ECM Analog scaffold
- De novo angiogenesis in ECM Analog … see figure
- In vivo cell proliferation
- Other studies are on going
- Injectable formulation of ECM Analog
- Development of a non-toxic quick gelling mechanism
- Cyto-compatibility of the formulation
- Proliferation of cells in injectable formulation
We therefore, believe that our biomaterial technology and proposal is novel and addresses all major critical questions through experimental demonstrations.